| BTEC 10 | BIOTECHNOLOGY: GENERAL PRINCIPLES | 5 Units |
| Introduction to the field of biotechnology. Topics covered include history, applications of biotechnology (in health care, agriculture, forensics, and the study of evolution), bioethics, the scientific method, lab safety and record-keeping, and instrumentation. Instruction in cell and molecular biology. May include field trips and/or presentations by industry speakers. Will allow for career exploration. |
| Advisory: Recent high school algebra or MATH 220; high school biology; eligibility for ENGL 1A or ESL 26. |
| Four hours lecture, one hour lecture-laboratory, two hours laboratory. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 51A | CELL BIOLOGY FOR BIOTECHNOLOGY | 3 Units |
| Introduction to cell biology. Topics to include cellular and subcellular structure, cellular metabolism, DNA replication, transcription and translation. |
| Three hours lecture. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 51AL | CELL BIOLOGY LABORATORY FOR BIOTECHNOLOGY | 5.5 Units |
| Introduction to the biological laboratory techniques and methods used in cell biology. Topics to include solution preparation, use of pH meters, cellular fractionation by centrifugation, enzymology, spectrophotometry, chromatography, microscopy, and electrophoresis. Laboratory exercises will also reinforce scientific method, lab safety, importance of laboratory notebooks, applied problem solving, and fundamentals of instrumentation. |
| Prerequisites: High school algebra or MATH 220; eligibility for ENGL 110 or ESL 25; high school biology or BIOL 10; CHEM 30A and 30B (or equivalent); or instructor permission. |
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| Two hours lecture, ten hours laboratory. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 52A | MOLECULAR BIOLOGY FOR BIOTECHNOLOGY | 3 Units |
| Introduction to molecular biology. Topics to include organization of the genome, control of gene expression, oncogenes, molecular events of the cell cycle, theory and applications of recombinant DNA technology. |
| Three hours lecture. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 52AL | MOLECULAR BIOLOGY LABORATORY FOR BIOTECHNOLOGY | 5.5 Units |
| Introduction to the biological laboratory techniques and methods used in molecular biology. Topics to include media preparation, agarose gel electrophoresis, restriction enzyme digestion, transformation of cells, purification and analysis of DNA, PCR, and Southern blotting. Laboratory exercises will also reinforce scientific method, lab safety, importance of laboratory notebooks, applied problem solving, and fundamentals of instrumentation. |
| Prerequisites: BTEC 51AL, or instructor permission. |
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| Two hours lecture, ten hours laboratory. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 53A | IMMUNOLOGY & VIROLOGY FOR BIOTECHNOLOGY | 3 Units |
| Introduction to immunology and virology. Topics to include the structure, function, and development of the immune system, regulation of the immune response, diseases of the immune system, vaccines, cancer, immunological techniques used in industry, viral structure, viral diseases, and the uses of viruses in biotechnology. |
| Prerequisites: BTEC 52A. |
| Three hours lecture. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 53AL | IMMUNOLOGY LABORATORY FOR BIOTECHNOLOGY | 5.5 Units |
| Introduction to the biological laboratory techniques and methods used in immunology. Topics to include the use of antibodies (ELISA, Western blot, immunofluorescence) in the lab, mammalian cell culture, and antibody production using hybridoma technology. Laboratory exercises will also reinforce scientific method, lab safety, importance of laboratory notebooks, applied problem solving, and fundamentals of instrumentation. |
| Prerequisites: BTEC 52AL, or instructor permission. |
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| Two hours lecture, ten hours laboratory. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 54 | BIOTECHNOLOGY EXTERNSHIP | 4 Units |
| Externship for Spring Quarter Biotechnology Technician Training Program students, arranged at biotechnology, pharmaceutical, instrumentation companies and research facilities. Provides applied learning experience in several diverse employment situations including, but not limited to, the areas of production, research and development, manufacturing and quality control. |
| Prerequisites: BTEC 52A & 52AL. |
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| Twenty-four hours clinic. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 60 | PLANT BIOTECHNOLOGY & MICRO-PROPAGATION | 3 Units |
| Introduction to current topics in plant propagation using modern biotechnology and micro-propagation. Topics include: 1) history of micro-propagation, 2) current trends in plant biotechnology including policy issues regarding unintended gene flow between plants, 3) principles of micro-propagation, 4) culture media and facilities, 5) preparation of culture media, and 6) techniques for micro-propagation (from seed to greenhouse). |
| Advisory: BIOL 10 or HORT 50A strongly recommended. |
| Two hours lecture, three hours laboratory. |
| GE Area: Non-GE Applicable |
| View Schedule. |
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| BTEC 61 | MICROBIAL BIOTECHNOLOGY | 4.5 Units |
| Introduction to microbiology with an emphasis on a practical approach to the utilization of microorganisms in biotechnology. Topics to include the current status of microbial biotechnology and potential contributions within a variety of fields, the genetic and biochemical diversity of microorganisms, their classification and metabolism, methods used to create engineered microorganisms, and the most widely exploited attributes of engineered microorganisms. |
| Advisory: BIOL 10 or HORT 50A strongly recommended. |
| Two hours lecture, two hours lecture-laboratory, four hours laboratory. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 64 | PROTEIN ELECTROPHORETIC SYSTEMS: BASIC LABORATORY TECHNIQUE | 1 Unit |
| Understanding, using, and performing electrophoretic separations and transfers in a research or industrial setting. This is to include the molecular and physical basis of specific techniques, and their practical applications. Techniques covered will include gel electrophoresis, capillary electrophoresis, isoelectric focusing, 2D gels and electrotransfers. The applications of these techniques for proteins, carbohydrates and small molecules, within research and industry will be presented. The instrumentation used for electrophoresis, isoelectric focusing, and capillary electrophoresis and practical experience with reagents and instrumentation will be emphasized. Students will follow established protocols, and demonstrate an understanding of supporting routine operations and standard protocols. |
| Prerequisites: Laboratory experience (high school and/or professional experience). |
| Advisory: BTEC 64 and BIOL 64 are interchangeable. High school biology, chemistry, and algebra recommended. |
| Repeatability: May be taken two times for credit. |
| Two hours lecture-laboratory. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 65 | NUCLEIC ACIDS ELECTROPHORETIC SYSTEMS: BASIC LABORATORY TECHNIQUE | 1 Unit |
| Understanding, using, and performing electrophoretic separations and transfers in a research or industrial setting. This is to include the molecular and physical basis of specific techniques, and their practical applications. Techniques covered will include gel electrophoresis, capillary electrophoresis and electrotransfers. The applications of these techniques for proteins, and small molecules, within research and industry will be presented. The instrumentation used for electrophoresis, capillary electrophoresis, and pulsed gel electrophoresis and practical experience with reagents and instrumentation will be emphasized. Students will follow established protocols, and demonstrate an understanding of supporting routine operations and standard protocols. |
| Prerequisites: Laboratory experience (high school and/or professional experience). |
| Advisory: BTEC 65 and BIOL 65 are interchangeable. High school biology, chemistry, and algebra recommended. |
| Repeatability: May be taken two times for credit. |
| Two hours lecture-laboratory. |
| GE Area: Non-GE Applicable |
| View Schedule. |
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| BTEC 66 | HPLC: BASIC LABORATORY TECHNIQUE | 2 Units |
| Understanding, using and performing HPLC in a research or industrial setting. Includes the theory and mechanisms of molecules and chemistry, the wide range of research, analytical and preparative uses, instrumentation used for HPLC, practical experience with reagents and instrumentation, following established protocols, calibrating and maintaining the instrumentation. |
| Prerequisites: High school biology, chemistry and algebra; laboratory experience. |
| Advisory: BTEC 66 and BIOL 66 are interchangeable. |
| Repeatability: May be taken two times for credit. |
| Four hours lecture-laboratory. |
| GE Area: Non-GE Applicable |
| View Schedule. |
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| BTEC 67 | IMMUNOLOGICAL ASSAYS | 1 Unit |
| Understanding and performing immunological assays. Includes the theory, molecular basis, and research/diagnostic applications of several techniques. Techniques covered will include, direct, indirect, sandwich, and quantitative ELISAs, and Western blotting. Practical experience with reagents (selection of conjugated antibodies, detection systems) and instrumentation (microtiter plate reader, polyacrylamide gel electrophoresis apparatus, transfer apparatus) will be emphasized. |
| Prerequisites: Laboratory experience (high school, college and/or professional); high school chemistry, biology, algebra. |
| Advisory: BTEC 67 and BIOL 67 are interchangeable. |
| Repeatability: May be taken two times for credit. |
| Two hours lecture-laboratory. |
| GE Area: Non-GE Applicable |
| View Schedule. |
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| BTEC 68 | POLYMERASE CHAIN REACTION: BASIC LABORATORY TECHNIQUE | 1 Unit |
| Understanding, using and performing PCR in a research or industrial setting. Includes the molecular and physical basis of the technique, mechanisms and practical (research and analytical) applications, RT-PCR, product separation and detection, thermocyclers, primers, practical experience with reagents and instrumentation for PCR, following established protocols. |
| Prerequisites: Laboratory experience (high school, college and/or professional); high school chemistry, biology, algebra. |
| Advisory: BTEC 68 and BIOL 78 are interchangeable. |
| Repeatability: May be taken two times for credit. |
| Two hours lecture-laboratory. |
| GE Area: Non-GE Applicable |
| View Schedule. |
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| BTEC 69 | BASIC MAMMALIAN CELL CULTURE TECHNIQUES | 3 Units |
| Introduction to general mammalian cell culture techniques, including media preparation, sterile technique, freezing, thawing, and maintaining primary cell and cell lines. Theoretical considerations will include purpose and selection of media components, setting up and maintaining a sterile cell culture environment, and controlling contamination. Students will gain practical experience working in the laminar flow hood, counting cells, isolating cells from a primary source, and maintaining healthy adherent and suspension cells in culture. Emphasis will also be given to proper care and use of equipment used in a cell culture facility: laminar flow hoods, CO2 incubators, water baths, and the inverted microscope. |
| Prerequisites: Laboratory experience (high school, college and/or professional). |
| Advisory: High school chemistry, biology, algebra recommended. |
| Repeatability: May be taken two times for credit. |
| Six hours lecture-laboratory. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 71 | DNA SEQUENCING & BIOINFORMATICS BASIC LABORATORY TECHNIQUES | 2 Units |
| Understanding, using and performing DNA sequencing and cloning techniques in a research and production setting. Includes applications of cDNA and PCR product sequencing, historical and theoretical basis of conventional and automated DNA sequencing, experimental design of sequencing methods, oligonucleotide synthesis, construction of sequencing and expressions plasmids, and vectorology. Laboratory exercises will involve DNA and RNA manipulation using established protocols and computer assisted methods (bioinformatics). |
| Prerequisites: Laboratory experience (high school and/or professional experience). |
| Advisory: BTEC 71 and BIOL 71 are interchangeable. High school biology, chemistry, and algebra recommended. |
| Repeatability: May be taken two times for credit. |
| Four hours lecture-laboratory. |
| GE Area: Non-GE Applicable |
| View Schedule. |
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| BTEC 73 | HISTOTECHNOLOGY IN RESEARCH | 1 Unit |
| Introduction to basic histotechnology techniques, including fixation, processing, embedding, sectioning, and staining. The course will stress hands-on work cutting thick and thin sections and individual staining techniques, including mixing all necessary solutions. The impact of histology as an aid in disease detection and how it is used as a tool in research will be explored. The course also addresses safety in the laboratory and ergonomic considerations along with an understanding of equipment maintenance. |
| Prerequisites: Laboratory experience (high school and/or professional experience). |
| Advisory: BTEC 73 and BIOL 73 are interchangeable. High school biology, chemistry, algebra recommended. |
| Repeatability: May be taken two times for credit. |
| Two hours lecture-laboratory. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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| BTEC 75 | IMMUNOBIOTECHNOLOGY: BASIC LABORATORY THEORY | 2 Units |
| Understanding immunobiology in relation to biotechnology. Introduction to molecular pathways associated with the human immune system. Inflammation, apoptosis, hematopoiesis, cellular activation, cellular genetics, signal transduction, and molecular classification in relation to current research in immunology. Discussion of current research trends in biotechnology with respect to the biology of the immune system. |
| Prerequisite: Laboratory experience. |
| Advisory: BTEC 75 and BIOL 75 are interchangeable. High school biology, chemistry, and algebra recommended. |
| Repeatability: May be taken two times for credit. |
| Two hours lecture. |
| GE Area: Non-GE Applicable |
| Not currently Scheduled. |
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